A fluorescent probe for specific detection of β-galactosidase in living cells and tissues based on ESIPT mechanism.

Jan 2, 2020

Contributor: Zihong Li,Mingguang Ren,Yuping Zhao,Wenhui Song,Jie Cheng,Weiying Lin

β-galactosidase is of great significance to living organisms, which is an important marker of primary ovarian cancer and cellular senescence. To detect the activity of β-galactosidase, a novel fluorescent probe ESIPT-GAL which based on excited state intramolecular proton transfer (ESIPT) mechanism for detecting β-galactosidase is developed in this work with low background fluorescence and high sensitivity (Φ = 0.0045-0.2409). The fluorescence intensity at 552 nm of this probe increased by ~ 55 times with β-galactosidase addition (0-4 U/mL), and its detection limit is very low (3.9 × 10 U/mL). In addition, the spectral data (pseudo-first-order rate: 1.303 min) and enzyme kinetic parameter (V = 69.5 μΜ•S) both show that the probe can achieve rapid response to β-galactosidase. Moreover, the probe has good water solubility, which ensures that it has good biocompatibility and can be easily applied to detect β-galactosidase in living cells and tissues. Importantly, the probe ESIPT-GAL can monitor β-galactosidase in deep mouse tissue sections (90 μm).

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About The Expert

Zihong Li

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China; Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China.

Mingguang Ren

Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China; Institute of State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Jinan 250353, PR China.

Yuping Zhao

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.

Wenhui Song

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China; Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China.

Jie Cheng

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.

Weiying Lin

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China; Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China. Electronic address: weiyinglin2013@163.com.

References

PubMed