Chronic exposure to diesel exhaust particulate matter impairs meiotic progression during spermatogenesis in a mouse model.

Jun 25, 2020

Contributor: Wei Yang,Yanyi Xu,Hongjie Pan,Fang Tian,Yuzhu Wang,Minjie Xia,Jingying Hu,Mingjun Yang,Shimin Tao,Shenfei Sun,Haidong Kan,Runsheng Li,Zhekang Ying,Weihua Li

Chronic exposure to diesel exhaust particulate matter impairs meiotic progression during spermatogenesis in a mouse model.

Exposure to ambient PM may correlate with the decline of semen quality, and the underlying biological mechanism has not been fully understood. In the present study, mice were intratracheally instilled with diesel exhaust PM (DEP), and its effects on the spermatogenic process as well as the alterations of testicular gene expression profile were assessed. Our results showed that chronic exposure to DEP impaired the fertility of male mice without influencing their libido. Compared with Vehicle-exposed group, the sperm count and motility from DEP-exposed mice were significantly decreased. In addition, immunohistological staining of γH2AX and DMC1, biomarkers for meiotic double strand breaks (DSBs), demonstrated that chronic exposure to DEP comprised the repair of meiotic DSBs, thus disrupting the spermatogenesis. Deep RNA sequencing test showed altered expressions of testicular genes including the GnRH signaling pathway. In summary, our research demonstrated that chronic exposure to DEP may disrupt spermatogenesis through targeting the meiotic recombination, providing a new perspective for the research on the male reproductive system damage caused by air pollution.
Copyright © 2020 Elsevier Inc. All rights reserved.

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About The Expert

Wei Yang

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: 17211150007@fudan.edu.cn.

Yanyi Xu

Department of Environmental Health, School of Public Health, Fudan University, Shanghai, 200032, China. Electronic address: xu@fudan.edu.cn.

Hongjie Pan

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: phjie1994@163.com.

Fang Tian

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: fannietian@qq.com.

Yuzhu Wang

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: 466959008@qq.com.

Minjie Xia

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: mito1983@163.com.

Jingying Hu

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: hujingying@aliyun.com.

Mingjun Yang

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: mingjun0707@163.com.

Shimin Tao

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: 18111150003@fudan.edu.cn.

Shenfei Sun

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: sunshenfei@tom.com.

Haidong Kan

Department of Environmental Health, School of Public Health, Fudan University, Shanghai, 200032, China. Electronic address: kanh@fudan.edu.cn.

Runsheng Li

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: runshengli2007@163.com.

Zhekang Ying

Department of Medicine Cardiology Division, University of Maryland School of Medicine, Baltimore, MD, 21201, USA. Electronic address: yingzhekang@hotmail.com.

Weihua Li

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Public Health, Fudan University, Shanghai, China. Electronic address: weihua.li@sippr.org.cn.

References

PubMed