The vaccinia virus based Sementis Copenhagen Vector vaccine against Zika and chikungunya is immunogenic in non-human primates.

Feb 11, 2021

Contributor: Natalie A Prow,Liang Liu,Mary K McCarthy,Kevin Walters,Raj Kalkeri,Jillian Geiger,Fusataka Koide,Tamara H Cooper,Preethi Eldi,Eri Nakayama,Kerrilyn R Diener,Paul M Howley,John D Hayball,Thomas E Morrison,Andreas Suhrbier

The Sementis Copenhagen Vector (SCV) is a new vaccinia virus-derived, multiplication-defective, vaccine technology assessed herein in non-human primates. Indian rhesus macaques (Macaca mulatta) were vaccinated with a multi-pathogen recombinant SCV vaccine encoding the structural polyproteins of both Zika virus (ZIKV) and chikungunya virus (CHIKV). After one vaccination, neutralising antibody responses to ZIKV and four strains of CHIKV, representative of distinct viral genotypes, were generated. A second vaccination resulted in significant boosting of neutralising antibody responses to ZIKV and CHIKV. Following challenge with ZIKV, SCV-ZIKA/CHIK-vaccinated animals showed significant reductions in viremias compared with animals that had received a control SCV vaccine. Two SCV vaccinations also generated neutralising and IgG ELISA antibody responses to vaccinia virus. These results demonstrate effective induction of immunity in non-human primates by a recombinant SCV vaccine and illustrates the utility of SCV as a multi-disease vaccine platform capable of delivering multiple large immunogens.

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About The Expert

Natalie A Prow

Inflammation Biology, QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4029, Australia.

Australian Infectious Disease Research Centre, Brisbane, QLD, 4029 and 4072, Australia.

Experimental Therapeutics Laboratory, School of Pharmacy and Medical Sciences, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, 5000, Australia.

Liang Liu

Experimental Therapeutics Laboratory, School of Pharmacy and Medical Sciences, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, 5000, Australia.

Mary K McCarthy

Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, 80045, USA.

Kevin Walters

Department of Infectious Disease Research, Southern Research Institute, Frederick, MD, 21701, USA.

Raj Kalkeri

Department of Infectious Disease Research, Southern Research Institute, Frederick, MD, 21701, USA.

Jillian Geiger

Department of Infectious Disease Research, Southern Research Institute, Frederick, MD, 21701, USA.

Fusataka Koide

Department of Infectious Disease Research, Southern Research Institute, Frederick, MD, 21701, USA.

Tamara H Cooper

Experimental Therapeutics Laboratory, School of Pharmacy and Medical Sciences, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, 5000, Australia.

Preethi Eldi

Experimental Therapeutics Laboratory, School of Pharmacy and Medical Sciences, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, 5000, Australia.

Eri Nakayama

Inflammation Biology, QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4029, Australia.

Department of Virology I, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan.

Kerrilyn R Diener

Experimental Therapeutics Laboratory, School of Pharmacy and Medical Sciences, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, 5000, Australia.

Robinson Research Institute and Adelaide Medical School, University of Adelaide, Adelaide, SA, 5005, Australia.

Paul M Howley

Sementis Ltd, Berwick, VIC, 3806, Australia.

John D Hayball

Experimental Therapeutics Laboratory, School of Pharmacy and Medical Sciences, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, 5000, Australia. john.hayball@unisa.edu.au.

Thomas E Morrison

Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, 80045, USA.

Andreas Suhrbier

Inflammation Biology, QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4029, Australia. Andreas.Suhrbier@qimrberghofer.edu.au.

Australian Infectious Disease Research Centre, Brisbane, QLD, 4029 and 4072, Australia. Andreas.Suhrbier@qimrberghofer.edu.au.

References

PubMed