Geometric transformation adaptive optics (GTAO) for volumetric deep brain imaging through gradient-index lenses.

Feb 05, 2024

Experts: Yuting Li, Zongyue Cheng, Chenmao Wang, Jianian Lin, Hehai Jiang, Meng Cui

The advance of genetic function indicators has enabled the observation of neuronal activities at single-cell resolutions. A major challenge for the applications on mammalian brains is the limited optical access depth. Currently, the method of choice to access deep brain structures is to insert miniature optical components. Among these validated miniature optics, the gradient-index (GRIN) lens has been widely employed for its compactness and simplicity. However, due to strong fourth-order astigmatism, GRIN lenses suffer from a small imaging field of view, which severely limits the measurement throughput and success rate. To overcome these challenges, we developed geometric transformation adaptive optics (GTAO), which enables adaptable achromatic large-volume correction through GRIN lenses. We demonstrate its major advances through in vivo structural and functional imaging of mouse brains. The results suggest that GTAO can serve as a versatile solution to enable large-volume recording of deep brain structures and activities through GRIN lenses.
© 2024. The Author(s).

Author

admin

View all posts

ABOUT THE EXPERTS

Yuting Li, Zongyue Cheng, Chenmao Wang, Jianian Lin, Hehai Jiang, Meng Cui

Yuting Li

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.

Zongyue Cheng

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.

Chenmao Wang

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.

Jianian Lin

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.

Hehai Jiang

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.

Meng Cui

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA. mengcui@purdue.edu.

Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA. mengcui@purdue.edu.

Department of Biology, Purdue University, West Lafayette, IN, 47907, USA. mengcui@purdue.edu.