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The following is a summary of “A clot waveform analysis-based system for differential diagnosis of prolonged activated partial thromboplastin time in plasma samples,” published in the December 2024 issue of Hematology by Shimonishi et al.
Prolonged activated partial thromboplastin time (aPTT) requires swift and precise differential diagnosis to identify underlying causes. Clot waveform analysis (CWA) provides an effective tool for diagnosing factor (F) VIII deficiency and distinguishing related conditions.
Researchers conducted a retrospective study to develop an accurate system for diagnosing aPTT prolongation, including FIX deficiency, using CWA-based mixing tests and template matching.
They incubated FVIII deficiency (FD) (n=96), lupus anticoagulant (LA) (n=19), and FVIII inhibitor (Inh) (n=28) samples with normal plasma and differentiated them using a mixing test and CWA-based template matching.
The results showed that in the mixing test, sensitivity for FD, Inh, and LA was 100%, 93%, and 100%, respectively, and specificity was 96%, 100%, and 100%. FIX inhibitor samples (>0.6 BU/mL) were identified as inhibitor samples. In template matching, nearly all severe hemophilia A and B cases were correctly classified.
Investigators developed a CWA-based system that aided in the differential diagnosis of prolonged aPTT. The system accurately distinguished between different causes of aPTT prolongation.
Source: link.springer.com/article/10.1007/s12185-024-03883-0
