Photo Credit: ChrisChrisW
The following is a summary of “Beneficial Alterations of Intestinal Microbiota in Chronic Cholecystitis Patients Treated With NOTES Gallbladder-Preserving Surgery,” published in the November 2024 issue of Gastroenterology by Deng et al.
People with chronic cholecystitis often experience altered gut microbiota. Natural Orifice Translumenal Endoscopic Surgery (NOTES) gallbladder-preserving surgery (N-GPS) has been proposed as a minimally invasive treatment for this condition.
Researchers conducted a prospective study to evaluate the impact of N-GPS on the intestinal microbiota of people with chronic cholecystitis.
They collected stool samples from HC group and people with benign gallbladder disease (BG group) at 2 stages, preoperative (BG_DPR stage) and 1 year postoperative (BG_YPO stage). The samples were analyzed for 16S ribosomal DNA (rDNA) sequencing using the Illumina MiSeq platform and short-chain fatty acids (SCFAs) with gas chromatography-mass spectrometry (GC-MS).
The results showed that the alpha diversity of the Shannon index did not show significant differences among the 2 stages of the BG group and the HC group. However, beta diversity based on the weighted UniFrac distance suggested that the intestinal microbiota structure at the BG_YPO stage was closer to the HC group. Linear discriminant analysis effect size (LEfSe) indicated that the BG_YPO stage enriched genera, such as Enterocloster and Hungatella_A_128155, are associated with improved bile acid metabolism. Additionally, compared to the BG_DPR stage, the BG_YPO stage and HC group showed enrichment in Faecalibacterium and Roseburia, while Streptococcus was depleted. Fecal SCFA concentrations also increased.
They concluded that people with chronic cholecystitis who underwent N-GPS showed improved gut microbiota structure, increased SCFA-producing bacteria, and improved bile acid metabolism.
Source: onlinelibrary.wiley.com/doi/full/10.1155/2024/9327118
