Using high resolution (400 MHz) magic angle spinning (HR-MAS) proton spectroscopy, the tissue metabolomic features linked with endometrial carcinoma (EC) at different grades were investigated.
The metabolic profiles of 64 individuals (14 with grade 1 (G1), 33 with grade 2 (G2), and 17 with grade 3 (G3) tumors) were compared to those of 10 patients with benign diseases. In comparison to non-transformed tissue, OPLS-DA demonstrated increased valine, isoleucine, leucine, hypotaurine, serine, lysine, ethanolamine, choline, and reduced creatine, creatinine, glutathione, ascorbate, glutamate, phosphoethanolamine, and scylloinositol in all EC classes. Higher taurine levels were also seen in the G1 and G2 tumors as compared to control tissue, as well as increased glycine, N-acetyl compound, and lactate levels in the G1 and G3 tumors.
Increased dimethyl sulfone, phosphocholine, and reduced glycerophosphocholine and glutamine levels are typical of G1 tumors, but decreasing myo-inositol levels are indicative of G2 and G3 cancers. The G3 tumors had higher amounts of 3-hydroxybutyrate, alanine, and betaine. The variations between grade G1 and G3 malignancies were mostly due to changes in phosphoethanolamine and phosphocholine production, as well as inositol, betaine, serine, and glycine metabolism. The univariate analysis confirmed the statistical significance of the OPLS-DA modeling. Metabolomics based on HR-MAS NMR gives helpful insight into metabolic reprogramming in endometrial cancer.
Reference:www.nature.com/articles/s41598-021-97505-y
