Intraoperative flow cytometry (iFC) was an effective tool for characterizing non-melanoma skin cancer (NMSC), according to a study in Cancers. Georgios Markopoulos, PhD, and col – leagues designed a prospective clinical feasibility study to evaluate iFC for the rapid diagnosis and complete surgical clearance of NMSC. The study included 30 patients diagnosed with NMSC. The iFC method demonstrated high sensitivity (95.2%), specificity (87.1%), and accuracy (91.1%), as con – firmed by receiver operating characteristic curve analysis. Most tumors were diploid, with two cases identified as hypoploid. The average G0/G1 fractions for normal and tumor tissue samples were 96.03±0.30% and 88.03±1.29%, respectively, with the tumor index increasing from 3.89±0.30% to 11.95±1.29% in cancerous cells. The findings highlight iFC’s potential to accurately characterize NMSC and evaluate surgical margins intraoperatively, which may improve complete tumor excision rates.