Liquid NanoBiosensors Enable One-Pot Electrochemical Detection of Bacteria in Complex Matrices.

Apr 24, 2023

Experts: Sara M Imani,Enas Osman,Fatemeh Bakhshandeh,Shuwen Qian,Sadman Sakib,Michael MacDonald,Mark Gaskin,Igor Zhitomirsky,Deborah Yamamura,Yingfu Li,Tohid F Didar,Leyla Soleymani

There is a need for point-of-care bacterial sensing and identification technologies that are rapid and simple to operate. Technologies that do not rely on growth cultures, nucleic acid amplification, step-wise reagent addition, and complex sample processing are the key for meeting this need. Herein, multiple materials technologies are integrated for overcoming the obstacles in creating rapid and one-pot bacterial sensing platforms. Liquid-infused nanoelectrodes are developed for reducing nonspecific binding on the transducer surface; bacterium-specific RNA-cleaving DNAzymes are used for bacterial identification; and redox DNA barcodes embedded into DNAzymes are used for binding-induced electrochemical signal transduction. The resultant single-step and one-pot assay demonstrates a limit-of-detection of 10 CFU mL , with high specificity in identifying Escherichia coli amongst other Gram positive and negative bacteria including Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus subtilis. Additionally, this assay is evaluated for analyzing 31 clinically obtained urine samples, demonstrating a clinical sensitivity of 100% and specify of 100%. When challenging this assay with nine clinical blood cultures, E. coli-positive and E. coli-negative samples can be distinguished with a probability of p < 0.001.
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.

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ABOUT THE EXPERTS

Sara M Imani,Enas Osman,Fatemeh Bakhshandeh,Shuwen Qian,Sadman Sakib,Michael MacDonald,Mark Gaskin,Igor Zhitomirsky,Deborah Yamamura,Yingfu Li,Tohid F Didar,Leyla Soleymani

Sara M Imani

School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Enas Osman

School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Fatemeh Bakhshandeh

Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Shuwen Qian

Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L8, Canada.

Sadman Sakib

Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Michael MacDonald

Department of Materials Science and Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L8, Canada.

Institute of Infectious Disease Research, McMaster University, Hamilton, ON, L8N 3Z5, Canada.

Mark Gaskin

Hamilton Regional Laboratory Medicine Program, Hamilton General Hospital, 237 Barton St. East, Hamilton, Ontario, L8L 2×2, Canada.

Igor Zhitomirsky

School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Department of Materials Science and Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L8, Canada.

Deborah Yamamura

Department of Pathology and Molecular Medicine, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.

Institute of Infectious Disease Research, McMaster University, Hamilton, ON, L8N 3Z5, Canada.

Yingfu Li

School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L8, Canada.

Institute of Infectious Disease Research, McMaster University, Hamilton, ON, L8N 3Z5, Canada.

Tohid F Didar

School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Department of Mechanical Engineering, McMaster University, Hamilton, ON, L8S 4L7, Canada.

Institute of Infectious Disease Research, McMaster University, Hamilton, ON, L8N 3Z5, Canada.

Leyla Soleymani

School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L7, Canada.

Institute of Infectious Disease Research, McMaster University, Hamilton, ON, L8N 3Z5, Canada.