Novel sheep model to assess critical-sized bone regeneration with periosteum for in-vivo bioreactors.

Feb 19, 2024

Experts: Yohaann Ghosh, Hai Xin, D S Abdullah Al Maruf, Kai Cheng, Innes Wise, Chris Burrows, Ruta Gupta, Veronica Ka-Yan Cheung, James Wykes, David Leinkram, Catriona Froggatt, Will Lewin, Hedi V Kruse, Eva Tomaskovic-Crook, David R McKenzie, Jeremy Micah Crook, Jonathan R Clark

Considerable research is being undertaken to develop novel biomaterials-based approaches for surgical reconstruction of bone defects. This extends to 3D printed materials that provide stable, structural, and functional support in vivo. However, few preclinical models can simulate in vivo human biological conditions for clinically relevant testing. Here we describe a novel ovine model that allows evaluation of in vivo osteogenesis via contact with bone and/or periosteum interfaced with printed polymer bioreactors loaded with biomaterial bone substitutes. The infraspinous scapular region of 14 Dorset-cross sheep was exposed. Vascularised periosteum was elevated either attached to the infraspinatus muscle or separately. In both cases, the periosteum was supplied by the periosteal branch of the circumflex scapular vessels. In eight sheep, a 3D printed 4-chambered polyetheretherketone (PEEK) bioreactor was wrapped circumferentially in vascularised periosteum. In six sheep, twelve double-sided 3D printed 2-chambered polyetherketone (PEK) bioreactors were secured to the underlying bone allowing direct contact with the bone on one side and periosteum on the other. Our model enabled simultaneous testing of up to 24 (12 double-sided) 10 x 10 x 5 mm bioreactors per scapula in the flat contact approach or a single 40 x 10 mm four-chambered bioreactor per scapula using the periosteal wrap. De novo bone growth was evaluated using histological and radiological analysis. Importantly, the experimental model was well tolerated by the animals and provides a versatile approach for comparing the osteogenic potential of cambium on the bone surface and elevated with periosteum. Furthermore, the periosteal flaps were sufficiently large for encasing bioreactors containing biomaterial bone substitutes for applications such as segmental mandibular reconstruction.

Author

admin

View all posts

ABOUT THE EXPERTS

Yohaann Ghosh, Hai Xin, D S Abdullah Al Maruf, Kai Cheng, Innes Wise, Chris Burrows, Ruta Gupta, Veronica Ka-Yan Cheung, James Wykes, David Leinkram, Catriona Froggatt, Will Lewin, Hedi V Kruse, Eva Tomaskovic-Crook, David R McKenzie, Jeremy Micah Crook, Jonathan R Clark

Yohaann Ghosh

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia.

Griffith University School of Medicine and Dentistry, 63619, Gold Coast, Queensland, Australia; yohaann.ghosh@lh.org.au.

Hai Xin

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia.

The University of Sydney Sydney Medical School, 7799, Sydney, New South Wales, Australia; hai.xin@sydney.edu.au.

D S Abdullah Al Maruf

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia.

The University of Sydney Sydney Medical School, 7799, Sydney, New South Wales, Australia; maruf.almaruf@lh.org.au.

Kai Cheng

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia.

Royal Prince Alfred Hospital, 2205, Institute of Academic Surgery, Camperdown, New South Wales, Australia; kai.cheng@health.nsw.gov.au.

Innes Wise

The University of Sydney Charles Perkins Centre, 560845, 5. Laboratory Animal Service, Sydney, New South Wales, Australia; innes.wise@sydney.edu.au.

Chris Burrows

The University of Sydney Charles Perkins Centre, 560845, 5. Laboratory Animal Service, Sydney, New South Wales, Australia; chris.burrows@sydney.edu.au.

Ruta Gupta

Royal Prince Alfred Hospital Department of Tissue Pathology and Diagnostic Oncology, 569326, Camperdown, New South Wales, Australia; ruta.gupta@health.nsw.gov.au.

Veronica Ka-Yan Cheung

Royal Prince Alfred Hospital Department of Tissue Pathology and Diagnostic Oncology, 569326, Camperdown, New South Wales, Australia; veronica.cheung@health.nsw.gov.au.

James Wykes

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia.

The University of Sydney Sydney Medical School, 7799, Sydney, New South Wales, Australia; james.wykes@lh.org.au.

David Leinkram

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia; dl@esoms.com.au.

Catriona Froggatt

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia; cate.froggatt@lh.org.au.

Will Lewin

Chris O’Brien Lifehouse, 10138, Arto Hardy Family Biomedical Innovation Hub, Camperdown, New South Wales, Australia.

The University of Sydney School of Medical Sciences, 216920, Sydney, New South Wales, Australia.

Chris O’Brien Lifehouse, 10138, Sarcoma Research Centre, Camperdown, New South Wales, Australia; will.lewin@lh.org.au.

Hedi V Kruse

Chris O’Brien Lifehouse, 10138, Arto Hardy Family Biomedical Innovation Hub, Camperdown, New South Wales, Australia.

The University of Sydney School of Physics, 144899, Sydney, New South Wales, Australia; hedi.kruse@lh.org.au.

Eva Tomaskovic-Crook

Chris O’Brien Lifehouse, 10138, 7. Arto Hardy Family Biomedical Innovation Hub, Camperdown, New South Wales, Australia.

The University of Sydney School of Medical Sciences, 216920, Sydney, New South Wales, Australia.

The University of Sydney School of Physics, 144899, Sydney, New South Wales, Australia; eva.tomaskoviccrook@lh.org.au.

David R McKenzie

Chris O’Brien Lifehouse, 10138, Arto Hardy Family Biomedical Innovation Hub, Camperdown, New South Wales, Australia.

The University of Sydney School of Physics, 144899, Sydney, New South Wales, Australia.

Chris O’Brien Lifehouse, 10138, Sarcoma and Surgical Research Centre, Camperdown, New South Wales, Australia; david.mckenzie@sydney.edu.au.

Jeremy Micah Crook

Chris O’Brien Lifehouse, 10138, Arto Hardy Family Biomedical Innovation Hub, Camperdown, New South Wales, Australia.

The University of Sydney School of Medical Sciences, 216920, Sydney, New South Wales, Australia.

Chris O’Brien Lifehouse, 10138, Sarcoma and Surgical Research Centre, Camperdown, New South Wales, Australia.

The University of Sydney School of Physics, 144899, Sydney, New South Wales, Australia; jeremy.crook@lh.org.au.

Jonathan R Clark

Chris O’Brien Lifehouse, 10138, Head and Neck Surgery, Camperdown, New South Wales, Australia.

The University of Sydney Sydney Medical School, 7799, Sydney, New South Wales, Australia.

Royal Prince Alfred Hospital, 2205, Institute of Academic Surgery, Camperdown, New South Wales, Australia; jonathan.clark@lh.org.au.