The aim of the study was to assess whether chronotype and subjective amplitude may predict suicidal ideation independently of mindfulness, and whether anxiety/insomnia and social dysfunction may be mediators of the relationship between chronotype and suicidal thoughts among medical students. The study group comprised 600 students of the medical faculties (191 men and 409 women), with a mean (SD, range) age of 21.94 (1.81, 18-31) years. The participants completed the Chronotype Questionnaire, the Five Facet Mindfulness Questionnaire (FFMQ) and the General Health Questionnaire (GHQ-28). Two items from GHQ-28 depression scale were extracted to measure suicidal ideation. The FFMQ score correlated negatively with the suicidal ideation score. The total effect of chronotype was insignificant when controlled for FFMQ. In the case of indirect effects, subjective amplitude score predicted suicidal ideation via both anxiety/insomnia and social dysfunction scores. The FFMQ score predicted suicidal ideation only via the social dysfunction scale. The direct effect of subjective amplitude was insignificant. Our findings indicate that the flexibility (or rigidity) of circadian rhythm may be linked to the intensity of experienced suicidal ideation, but only via anxiety/insomnia and social dysfunction, independently of mindfulness and morningness-eveningness.© 2023 European Sleep Research Society.