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The following is a summary of “Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light,” published in the March 2025 issue of Journal of Pain by Sapio et al. 

Autofluorescence in human tissue, particularly from lipofuscin (LF) in dorsal root ganglia (DRG), interferes with multiplex fluorescence assays, limiting their effectiveness. 

Researchers conducted a retrospective study to develop a scalable pre-staining, white-light photobleaching method to reduce LF autofluorescence and improve signal detection in multiplex fluorescence assays.  

They applied a pre-staining, white-light photobleaching method to human tissue samples, including peripheral and central nervous system structures and pathological tissues such as Alzheimer’s disease brain. The effectiveness of this technique in minimizing LF autofluorescence and maintaining signal detection across the color spectrum was assessed.  

The results showed that the photobleaching method reduced LF autofluorescence, improving signal detection without compromising multiplex fluorescence assays. The technique showed effectiveness across multiple human tissues, including those with high levels of autofluorescent interference.  

Investigators concluded a low-cost, scalable method enhances fluorescence detection in human tissue, thereby expanding high-plex microscopy’s use in translational research and improving disease investigations.

Source: jpain.org/article/S1526-5900(25)00586-3/fulltext